Stable expression of human aromatase complementary DNA in mammalian cells: a useful system for aromatase inhibitor screening.

Stable Expression of Human Aromatase Complementary DNA in Mammalian Cells: A Useful System for Aromatase Inhibitor Screening1

A mammalian cell expression plasmid, pH ,>'-\ro. containing the human placenta aromatase complementary DNA was constructed. The prepared plasmid was used to transfect breast cancer cells (MCF-7), noncancerous breast cells ( I lit] 1(10),and Chinese hamster ovary cells by a stable expression method. While the maximum velocities for aro matase expressed in three types of cells »eredifferent (10-...

P-227: Functional Analysis of The I.a,I.b, I.c and I.d (PII) Promoters of CYP19 (aromatase) Gene in Granulosa Cells of Polycystic Ovaries Patients and The Role of Letrozole and Antisensearom on CYP19 Gene Expression Inhibition

Background: The key enzyme of estrogen biosynthesis, aromatase cytochrome P450,is encoded by the CYP19 gene.CYP19 plays an important role in the development,function,an regulation of the female reproduction cycle. Thus, it is the potential candidate gene affecting fertility performance in human. CYP19 transcripts are expressed mainly in the ovary,testes,breast,adipose tissue and brain. Tissue e...

A yeast screen system for aromatase inhibitors and ligands for androgen receptor: yeast cells transformed with aromatase and androgen receptor.

Endocrine disruptors are hormone mimics that modify hormonal action in humans and animals. It is thought that some endocrine disruptors modify estrogen and androgen action in humans and animals by suppressing aromatase activity. Aromatase cytochrome P450 is the key enzyme that converts C19 androgens to aromatic C18 estrogenic steroids. We have developed a novel aromatase inhibitor screening met...

P-200: Effects of The ?3 Fatty Acid Eicosapentaenoic Acid on PPARa and Aromatase in Human Granulosa Cells

P-11: Differential Expression of mRNA Aromatase in Ejaculated Spermatozoa from Infertile Men in Relation to either Asthenozoospermia or Teratozoospermia

Background Estrogens biosynthesis in ejaculated spermatozoa is an autonomous process that may influence sperm functions. The purpose of this study was to evaluate the relationship between the expression of aromatase, sperm quality and seminal neutral α–glucosidase marker in semen of Tunisian infertile men. MaterialsAndMethods Fifty seven men were investigated: they were composed of two groups: ...